After being allowed back on Twitter, Trump posted two tweets on Friday that the company cited as the finals straws.
Twitter has suspended US President Donald Trump permanently from its platform "due to the risk of further incitement of violence".
The decision was made "after close review of recent Tweets from the @realDonaldTrump account", Twitter said.
It comes amid a Big Tech purge of the online platforms used by Trump and his supporters.
Some lawmakers and celebrities have been calling for years on Twitter to ban Trump altogether.
Former First Lady Michelle Obama tweeted on Thursday that the Silicon Valley giants should stop enabling Trump's "monstrous behaviour" and permanently expel him.
Trump was locked out of his account for 12 hours on Wednesday after he called the people who stormed the US Capitol "patriots".
Hundreds of his supporters entered the complex as the US Congress attempted to certify Joe Biden's victory in the presidential election. The ensuing violence led to the deaths of four civilians and a police officer.
Twitter warned then that it would ban Trump "permanently" if he breached the platform's rules again.
After being allowed back on Twitter, Trump posted two tweets on Friday that the company cited as the finals straws.
In one, he wrote: "The 75,000,000 great American Patriots who voted for me, AMERICA FIRST, and MAKE AMERICA GREAT AGAIN, will have a GIANT VOICE long into the future. They will not be disrespected or treated unfairly in any way, shape or form!!!"
Twitter said this tweet "is being interpreted as further indication that President Trump does not plan to facilitate an 'orderly transition'".
In the next, the president tweeted: "To all of those who have asked, I will not be going to the Inauguration on January 20th."
Twitter said this was "being received by a number of his supporters as further confirmation that the election was not legitimate".
Twitter said both of these tweets were "in violation of the Glorification of Violence Policy".
After Twitter had permanently suspended his @realDonaldTrump account, Trump tweeted from the US president's official @Potus account suggesting he would "look at the possibilities of building out our own platform in the future" and railing against Twitter.
But the tweets were removed from the platform as soon as they were posted.
Reacting to the ban, Trump 2020 campaign adviser Jason Miller tweeted: "Disgusting... if you don't think they're coming for you next, you're wrong."
Earlier on Friday, Twitter permanently banned the account of conservative radio host Rush Limbaugh and two Trump loyalists: former national security adviser Michael Flynn and attorney Sidney Powell.
Later in the day, Google suspended Parler - a self-styled "free speech" rival to Twitter that is increasingly popular with Trump supporters - from its online store.
"We're aware of continued posting in the Parler app that seeks to incite ongoing violence in the US," said Google.
On Thursday, Facebook said it had suspended Trump "indefinitely". The popular gaming platform Twitch also placed an indefinite ban on the outgoing president's channel, which he has used for rally broadcasts. So has Snapchat.
Two online Trump memorabilia stores were closed this week by e-commerce company Shopify. On Friday, Reddit banned its "donaldtrump" forum for the president's supporters.
Trump used Twitter to insult adversaries, cheer allies, fire officials, deny "fake news" and vent grievances, often using all capital letters and exclamation marks to underline his point.
Though critics said the posts were a torrent of misinformation, the medium helped him get around media filters and instantly connect with nearly 89 million followers.
His tweets were also known for the occasional spelling error, and he sometimes left followers guessing with apparent mis-types, such as when he posted, "Despite the constant negative press covfefe".The Department of Justice said in 2017 that Trump's tweets were "official statements of the President of the United States". - BBC, Source: https://www.daily-bangladesh.com